FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763235

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763235
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33993643
Sequences flagged as poor quality0
Sequence length100-125
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA609750.040.0027282
GATCGGA622200.039.6271251
GACCGTA367550.039.49760436-37
CGGAAGA625450.038.7205664
CGCGTAT81550.038.63935544-45
AAGACCG380850.038.16927734-35
TCGGAAG639050.038.0028083
ATCGCGT83700.037.8786542-43
CCGTATC397200.036.73429538-39
TATGCCG400400.036.11912548-49
TACGCAC339900.035.611317118-119
AGAGCAC684250.035.285998
GAGCACA679500.035.2756929
GTATCGC90750.035.17650640-41
CGTATGC418800.034.51515646-47
CTACGCA358350.034.05699116-117
TCTACGC368900.033.137245118-119
ATCTACG395750.033.06288118-119
CGTCTGA455900.033.06134416-17
TATCGCG81900.032.8466540-41
Produced by FastQC (version 0.11.5)