FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763244
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3618446
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG11950.060.7326743
CTACTCG11550.059.2306252
CGAGTAG11800.046.884733
CCGAGTA12550.045.504882
CTCGGGA17150.044.7461935
GGAGGCT80750.043.7609149
TCGGGAG18600.043.4967166
TTGGGAG80300.043.4122736
GCTGGGA100550.042.420879
ACTCGGG18050.041.5263524
GAGTAGC31450.041.235034
AGTAGCT50500.039.933635
GGGAGGC101900.039.9317558
CGGAGGT17750.039.547057
GTAGCTG53750.037.740396
CCCGAGT17900.036.2321361
TAGCTGG61950.034.7614367
TGGGAGG103150.034.4875567
GCACTTT71850.033.8701441
TTTGGGA84150.033.579175
Produced by FastQC (version 0.11.5)