FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4353013
Sequences flagged as poor quality0
Sequence length35-351
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT144300.086.327325
CGAGTAG44750.085.024983
GTAGCTG149500.082.726966
CCGAGTA47300.080.490172
GAGTAGC100100.077.211494
GCTGGGA298900.074.462679
TTGGGAG194700.070.826166
TAGCTGG180350.070.3537147
AAGTGCT143600.070.207395
CACGCTA6859.4989155E-567.4736320-329
CCCGAGT56700.066.734031
GCACTTT162650.064.513971
TTTGGGA181350.063.7635545
CAAGTAG64100.061.3330463
CTACTCG36750.061.022822
GGAGGCT200000.060.910469
AGTGCTG162250.060.8982546
AAGTAGC68500.059.354
ACTTTGG173700.058.9844133
CACTTTG173450.058.9765432
Produced by FastQC (version 0.11.5)