FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763246

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763246
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4353013
Sequences flagged as poor quality0
Sequence length35-251
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG37800.088.564713
CCGAGTA40350.086.293392
AGTAGCT138050.086.002385
GAGTAGC91200.083.948624
GTAGCTG144000.081.173586
TTGGGAG218150.076.018986
CTACTCG45600.075.7387542
GCTGGGA296750.074.456929
TACTCGG46600.074.265023
CCCGAGT48850.071.749821
AAGTGCT148550.071.032495
TTTGGGA188600.070.029195
GGAGGCT236200.069.410369
GCACTTT174250.069.1846851
GCTACTC100250.069.008411
TCGGGAG56500.067.753236
CTCGGGA51900.067.362315
TAGCTGG178450.067.249797
ACTTTGG178200.066.82273
CACTTTG183600.064.7803962
Produced by FastQC (version 0.11.5)