FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4080491
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG17300.061.556053
CTACTCG17700.060.164952
CGAGTAG17200.058.8009383
GCTACTC42750.058.3169671
CCGAGTA18400.056.2594152
GAGTAGC43900.051.9036674
AGTAGCT71400.051.660345
TTGGGAG111600.051.6575786
GCTGGGA141950.051.3016249
GGAGGCT113350.051.1762169
CCCGAGT21800.050.4931261
TCGGGAG23300.048.514566
GTAGCTG75400.047.8162736
CTCGGGA23700.044.4312675
GGGAGGC152400.044.231338
TAGCTGG83500.044.0328147
CCCAAAG109450.043.9794461
ACTCGGG23900.043.0637634
ACTTTGG94150.042.4632033
AAGTGCT90450.042.4243245
Produced by FastQC (version 0.11.5)