FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763251

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763251
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4325306
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG18800.064.559073
CCGAGTA19650.059.7077332
GAGTAGC48750.055.4071924
CTACTCG15100.054.823252
AGTAGCT78300.053.644835
GTAGCTG80750.051.2067456
TACTCGG16150.051.2067453
GCTACTC39550.049.3731271
CCCGAGT25850.048.97391
GCTGGGA164400.048.1348729
TAGCTGG92950.046.4086157
TTGGGAG111450.044.841916
GGAGGCT110400.043.1152539
AAGTGCT97300.040.5401735
CTCGGGA22000.039.7538875
GGGAGGC145600.039.5161938
TCGGGAG23950.039.249716
ACTCGGG22000.038.4017144
GCACTTT95750.037.85641
GTGCTGG101050.037.801477
Produced by FastQC (version 0.11.5)