FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3436552
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG14650.066.602213
CCGAGTA15650.062.788762
AGTAGCT57350.055.2937745
CCCGAGT20450.054.6321141
GTAGCTG59650.052.962266
GAGTAGC38550.051.701474
GCTGGGA121850.049.316359
TACTCGG11150.048.5566833
CTACTCG11350.047.7482952
TAGCTGG69900.047.3252267
GCTACTC30650.045.8075561
GGAGGCT84250.041.3830769
TTGGGAG87000.039.73196
AAGTGCT76700.039.7152255
AGTGCTG76800.039.6635136
GTGCTGG79600.038.568417
CCCAAAG95000.038.4881971
CGGAGGT17150.037.46757
AAAGTGC80850.037.0143744
GGGAGGC105700.036.3624048
Produced by FastQC (version 0.11.5)