FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763254

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763254
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3436552
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG13750.067.499673
CTACTCG13850.061.8575132
CCGAGTA15300.060.6614722
TACTCGG14750.060.099933
GCTACTC35600.057.168521
GAGTAGC35700.054.995294
CCCGAGT18600.052.1501461
GTAGCTG59250.051.913696
AGTAGCT58400.051.8542865
GCTGGGA124300.051.3131529
GGAGGCT96000.050.3876769
TTGGGAG96100.048.722676
CTCGGGA18200.047.726685
TAGCTGG70700.046.873587
TCGGGAG20800.046.337376
CGGAGGT24600.045.9526827
AAAGTGC78300.043.538414
ACTCGGG19600.043.4069944
GGGAGGC129250.043.3629728
CCCAAAG98150.043.0475961
Produced by FastQC (version 0.11.5)