FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763256

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763256
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4102139
Sequences flagged as poor quality0
Sequence length35-251
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT155150.094.5828865
CGAGTAG45100.093.6429753
CCGAGTA46900.092.025312
GTAGCTG161650.091.353396
GAGTAGC107900.087.339644
TTGGGAG242350.083.2760246
GCTGGGA340200.080.155639
AAGTGCT154400.079.517665
TTTGGGA210600.079.1306155
CCCGAGT56250.078.2925341
GCACTTT193950.077.54121
ACTTTGG199400.075.489643
TAGCTGG202950.074.984977
CACTTTG199700.074.625132
CTACTCG48450.073.915362
GGAGGCT251750.072.6084759
TACTCGG49250.072.425883
CAAGTAG61750.071.743853
AGTGCTG169450.071.697356
GCTACTC108450.071.278181
Produced by FastQC (version 0.11.5)