FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763257

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763257
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4717474
Sequences flagged as poor quality0
Sequence length35-351
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT162500.090.476755
GTAGCTG172500.085.547066
TTAACGC4150.001109684683.39698340-345
CGAGTAG50200.083.126563
CCGAGTA51650.081.291642
GAGTAGC119300.079.271914
GCTGGGA337400.075.9368069
TAGCTGG209350.072.9403847
TTGGGAG219800.071.592346
AAGTGCT157900.068.899355
CCCGAGT63600.067.96161
TTTGGGA200900.067.110245
GCACTTT196250.062.542371
GGAGGCT223850.062.4181679
TGAGTAG82000.062.2616653
CAAGTAG73600.060.076373
AGTGCTG177750.059.2815176
GGGAGGC315400.059.0095448
AAGTAGC77650.058.944474
ACTTTGG206650.058.8502273
Produced by FastQC (version 0.11.5)