FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763264

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763264
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3816991
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG18050.072.8371053
CCGAGTA18750.071.704232
CTACTCG20750.066.226452
GCTACTC48000.065.697961
GAGTAGC47650.065.669014
TACTCGG22100.062.719313
CCCGAGT23000.061.0522161
AGTAGCT78400.060.6281055
GTAGCTG80350.057.9743966
GCTGGGA163350.056.709829
TTGGGAG123800.054.4464576
GGAGGCT133500.053.167719
AAGTGCT93050.052.1695485
ACTCGGG27750.051.2356834
TAGCTGG92850.050.8122837
AAAGTGC94950.050.749024
TCGGGAG29850.050.6232156
CCCAAAG116700.049.710831
CTCGGGA28200.049.1530045
CCAAAGT107900.048.8483472
Produced by FastQC (version 0.11.5)