FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763265
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4744019
Sequences flagged as poor quality0
Sequence length35-351
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT194550.095.029145
GTAGCTG198300.092.319516
CGAGTAG62900.088.5852363
GAGTAGC138400.085.913844
CCGAGTA64700.084.06562
GCTGGGA394700.080.137729
TTGGGAG254050.077.491716
TAGCTGG243200.077.322347
AAGTGCT172300.077.193535
TTTGGGA227900.074.3075565
GCACTTT216050.072.446151
CAAGTAG78400.071.6486053
CCCGAGT79550.069.734121
CCAAGTA82900.068.794632
AGTGCTG189450.068.5340046
CTGAGTA88200.068.509572
ACTTTGG227350.067.688613
GGAGGCT256450.067.1530159
TGAGTAG90150.066.659213
CACTTTG230900.066.420292
Produced by FastQC (version 0.11.5)