FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763267

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763267
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4703658
Sequences flagged as poor quality0
Sequence length35-351
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG37600.075.9116443
AGTAGCT130800.075.8537455
GTAGCTG136950.072.552386
CCGAGTA39700.071.031442
GAGTAGC90050.068.822314
GCTGGGA270850.065.035769
TTGGGAG185500.063.2918746
TAGCTGG163200.061.7637337
CCCGAGT48350.059.340071
AAGTGCT136650.058.9269685
GGAGGCT186500.056.0532159
TTTGGGA175250.055.875935
GCACTTT161300.053.495991
CTACTCG29150.053.05852
CAAGTAG61650.052.5955663
AGTGCTG151750.052.3527226
GGGAGGC261000.051.815928
TACTCGG30950.051.8024023
ACTTTGG167100.051.4158673
GCTACTC71950.050.3962971
Produced by FastQC (version 0.11.5)