FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4703658
Sequences flagged as poor quality0
Sequence length35-251
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG32550.083.140353
CGCCGAA3503.999711E-580.3542240-244
CCGAGTA34900.078.9223862
AGTAGCT127000.078.892215
GAGTAGC80150.074.832374
CTAAGCG4254.048858E-473.7176245
GTAGCTG134550.073.0323266
TTGGGAG207050.069.443276
GCTGGGA279350.067.155889
CCCGAGT43600.065.421371
CTACTCG37600.065.013892
TACTCGG38550.063.7696573
AAGTGCT142500.063.2556955
GGAGGCT218450.062.7374279
TTTGGGA187650.062.604475
GCACTTT167300.062.432261
GCTACTC90750.062.4066661
TAGCTGG162600.061.963457
ACTTTGG171900.059.6871223
CACTTTG172850.059.5177962
Produced by FastQC (version 0.11.5)