FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4419617
Sequences flagged as poor quality0
Sequence length35-351
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATA4555.42395E-499.84742340-345
AGTAGCT122650.073.5280155
CGAGTAG32850.073.28193
GTAGCTG125950.070.9001166
CCGAGTA35250.069.802582
GAGTAGC81650.068.343514
GCTGGGA245250.063.9688539
TAGCTGG146300.062.0458687
TTGGGAG175050.060.854226
CCCGAGT43050.058.1064221
GGAGGCT164600.057.6523749
AAGTGCT127400.056.9181945
TTTGGGA169550.053.2325175
CTACTCG29400.053.1217842
GGGAGGC231650.052.7239848
GCACTTT156250.051.900951
AGTGCTG140000.051.690396
TACTCGG30200.050.7038233
GCTACTC70500.049.9260141
ACTTTGG163500.049.6190153
Produced by FastQC (version 0.11.5)