FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4419617
Sequences flagged as poor quality0
Sequence length35-251
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATA4001.9365805E-592.99529245
CGAGTAG31400.084.772143
CCGAGTA33350.080.655082
AGTAGCT118350.080.055695
GTAGCTG123150.076.707236
GAGTAGC75900.075.692884
GCTGGGA251750.070.4752969
CTACTCG35100.069.630722
TACTCGG35850.068.764063
TTGGGAG187750.067.372436
CCCGAGT40600.066.2966461
GCTACTC82250.065.364861
TAGCTGG149000.065.334887
AAGTGCT137600.062.577835
GGAGGCT196450.062.1041769
GCACTTT155900.061.12691
CTCGGGA41900.060.8472675
TTTGGGA171750.060.4402355
TCGGGAG45300.060.001386
CAAGTAG57300.057.8536723
Produced by FastQC (version 0.11.5)