FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763275

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763275
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4471860
Sequences flagged as poor quality0
Sequence length35-351
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT124300.079.9385
GTAGCTG129300.076.388046
CGAGTAG35600.074.776323
GAGTAGC85100.069.707594
GCTGGGA259800.069.1898359
CCGAGTA38350.069.07392
TAGCTGG156900.064.845517
TTGGGAG181050.063.523286
AAGTGCT136500.061.4941025
CGCGTTA1900.00728692559.068993310-319
CCCGAGT47650.058.960311
AAGTCGT6700.00451261558.545612330-339
CTACTCG31100.058.2157552
GGAGGCT178500.057.9957249
TTTGGGA170850.056.8125535
CAAGTAG61650.055.4482083
AGTGCTG150100.055.0825236
TACTCGG33950.055.0402533
GCTACTC76250.054.1958241
GGGAGGC248700.054.0593458
Produced by FastQC (version 0.11.5)