FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3039940
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG12900.066.8763053
CCGAGTA13800.064.298592
GAGTAGC33250.057.9758834
AGTAGCT55800.054.1655965
CCCGAGT18400.050.2941861
GCTGGGA111050.049.935079
GTAGCTG60050.049.3412746
GCTACTC26350.047.3554041
TTGGGAG77450.045.7845426
TAGCTGG66400.045.698647
CTACTCG9950.045.4867362
TACTCGG10900.043.667423
GGAGGCT78250.042.3525129
AAGTGCT67350.040.9896665
CCCAAAG84750.040.0140531
GGGAGGC98400.039.726348
GCACTTT67200.039.5361751
AGTGCTG71100.039.329856
AAAGTGC69650.038.2693374
GTGCTGG72150.038.0984087
Produced by FastQC (version 0.11.5)