FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763278

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763278
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3039940
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG12750.064.859523
TACTCGG13600.062.9930653
GAGTAGC31700.062.496274
CTACTCG13450.061.9262662
GCTACTC32200.061.9126661
CCGAGTA13950.060.986122
AGTAGCT57200.055.6450965
GCTGGGA119150.055.075419
GTAGCTG58000.054.7756
CCCGAGT16950.053.7170681
TTGGGAG88400.053.5037236
GGAGGCT93800.053.4688729
CTACTTG26300.049.5401732
TAGCTGG67500.049.1820567
TCGGGAG19900.049.0297746
AAAGTGC73250.048.8130534
CCCAAAG90000.048.0709271
AAGTGCT73900.047.7396665
CCAAAGT83450.046.4825972
AGTGCTG75750.046.2595946
Produced by FastQC (version 0.11.5)