FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3493510
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG8800.037.1842963
CGTCGAG1452.57387E-937.0711021
CCGAGTA10500.035.7316932
TACTCGG9750.035.391823
TCGCGTT851.7120645E-434.996985
GCTGGGA81600.033.6870049
CTACTCG9550.033.673852
TTGGGAG62800.031.7369126
GGGAGGC72050.031.2979478
CGGAGGT11500.031.0425787
CTCGCGT1551.9742765E-730.7070314
GCTACTC23300.029.7346671
AGTAGCT44050.029.4435445
TAGCTGG49750.029.061477
GGAGGCT62700.029.037859
CCCGAGT14250.028.9197981
GTAGCTG45950.028.485036
TCGGGAG13250.028.2881286
GGAGGTT33300.027.1587628
CTCGGGA12900.026.7496325
Produced by FastQC (version 0.11.5)