FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763282

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763282
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3348050
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG13500.067.4248052
TACTCGG14000.064.166883
GAGTAGC32750.053.4069824
CGAGTAG13700.052.97873
AGTAGCT58000.052.414925
GCTGGGA119850.052.17469
TTGGGAG90350.050.5725146
CCGAGTA14850.049.677222
GTAGCTG59550.049.6542136
TCGGGAG20600.047.076256
GGAGGCT91950.046.911659
CGGAGGT20150.046.651277
ACTCGGG18750.046.6420974
CTCGGGA19350.046.4256485
CCCGAGT18050.045.8244131
AAGTGCT75800.045.6789175
AAAGTGC78000.044.8481754
CCCAAAG95050.044.5745741
TAGCTGG68800.044.534887
AGTGCTG79800.043.8385246
Produced by FastQC (version 0.11.5)