FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4501772
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG3550.035.4271621
CGAGTAG18050.031.764473
CCGAGTA19200.030.8480682
ACGTCGA3150.028.5184631
CCCGAGT23950.027.006211
GAGTAGC41550.026.4472064
TAGCTGG73650.024.7158577
GCTGGGA122550.024.6591079
AGTAGCT67500.024.3300295
GTAGCTG70950.023.1410836
CTACTCG13700.020.5244392
TTGGGAG87300.020.510646
CCCAAAG93100.020.1987921
GGAGGCT92000.019.5274739
GGGAGGC107050.019.2272178
GCTACTC33900.018.9029461
TACTCGG15400.018.6152173
AGCTGGG103100.018.2328858
AAAGTGC82450.018.1084964
CCAAAGT91300.018.0855732
Produced by FastQC (version 0.11.5)