FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763284

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763284
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4501772
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG2056.91216E-1131.9654371
CGAGTAG15400.027.0519833
GAGTAGC38600.026.3651714
AGTAGCT63300.025.853365
TACTCGG16750.025.5822933
CCGAGTA16500.025.2485162
GCTACTC38850.025.147491
CTACTCG15650.025.0987072
GTAGCTG64550.024.60786
GCTGGGA124000.024.276539
TAGCTGG71950.024.2267177
TTGGGAG91350.022.7938256
CCCGAGT22000.022.4748731
GGAGGCT98450.022.116849
CTCGGGA19450.021.7233475
GGGAGGC113700.021.2433668
CGCGTAA853.9376257E-420.99911938-39
CGGAGGT18350.019.7765967
ACTCGGG22100.019.658594
AAAGTGC79250.019.2999254
Produced by FastQC (version 0.11.5)