FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763287

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763287
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2748006
Sequences flagged as poor quality0
Sequence length35-351
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAG4102.5087327E-480.063736310-319
CGAGTAG22700.078.274623
AGTAGCT85800.077.877295
CCGAGTA23350.075.6308752
GAGTAGC57300.073.630834
GTAGCTG91300.073.58836
TTGGGAG111750.068.264586
GCTGGGA173100.066.6390159
AAGTGCT78600.066.594935
TAGCTGG106350.065.168857
CCCGAGT28050.064.2524641
TTTGGGA106850.061.4435845
CAAGTAG37850.059.9941943
GGAGGCT112300.059.1571969
GCACTTT96800.059.124751
CTACTCG16800.057.6716962
GCTTTCG5550.004828253357.54696250-259
AAGTAGC39550.057.414384
CTATTCG3455.8279307E-557.39456340-345
AGTGCTG90100.057.103296
Produced by FastQC (version 0.11.5)