FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763288

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763288
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2748006
Sequences flagged as poor quality0
Sequence length35-251
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACG1300.008271151137.69412240-244
CGAGTAG22800.086.239753
AGTAGCT81050.083.9627155
CCGAGTA24100.082.959842
GAGTAGC52750.082.4572454
GTAGCTG85500.080.657596
GATCGGT1800.00267508476.7466245
TTGGGAG129750.073.961256
GCGTCGA1606.5761364E-572.68841235-239
GCTGGGA178500.071.707069
GCACTTT100950.070.54221
CCCGAGT28400.070.209361
CTACTCG20400.068.799252
AAGTGCT84150.068.204235
TTTGGGA116600.068.014995
GCTACTC52000.067.900151
ACTTTGG106600.066.76323
TAGCTGG108150.066.641537
TACTCGG21500.066.5121843
CACTTTG108300.065.591762
Produced by FastQC (version 0.11.5)