FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3325114
Sequences flagged as poor quality0
Sequence length35-351
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGA1200.001447665278.00559300-309
AGTAGCT85050.069.8569645
CGAGTAG24400.068.949353
GTAGCTG89000.066.8809366
GAGTAGC60150.066.559274
CCGAGTA25800.064.4826662
GCTGGGA172450.063.3695229
TAGCTGG101400.060.447217
TTGGGAG117250.060.364766
CTACTCG18600.059.2468952
AAGTGCT92500.058.0147745
TACTCGG20000.056.7886663
CGAAATA5900.002493228554.99983340-345
GGAGGCT120300.054.245249
GCTACTC45400.054.0669171
CCCGAGT30450.053.5077361
TTTGGGA112050.053.277365
GCGCGAG4950.00706030452.284473250-259
GCACTTT103900.051.7695771
GGGAGGC163400.051.7188228
Produced by FastQC (version 0.11.5)