FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3174598
Sequences flagged as poor quality0
Sequence length35-351
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTAA3200.0076718666141.1625330-339
GCGTATG3500.00812072138.4975340-345
ATTCGCG1609.134965E-879.84477250-259
CGAGTAG21900.075.319653
CCGAGTA22900.071.5489652
AGTAGCT83850.069.405325
GAGTAGC53150.068.24924
GTAGCTG84550.068.068086
GCTGGGA167050.063.381529
CCCGAGT27200.062.7672731
TAGCTGG97650.060.100247
CTACTCG16300.059.7350732
TTGGGAG109350.059.5379836
AAGTGCT84200.056.6949045
TACTCGG17800.055.7518163
GCTACTC39350.055.5145761
GGAGGCT111400.055.3822339
CCAAGTA37950.053.083372
CAAGTAG37150.052.7037243
CGAATAG4605.5932884E-552.689266340-345
Produced by FastQC (version 0.11.5)