FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763292

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763292
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3174598
Sequences flagged as poor quality0
Sequence length35-251
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGTT5854.149524E-6109.73403245
CGAGTAG19550.080.941993
CCGAGTA20550.077.636982
GAGTAGC48250.076.929324
AGTAGCT78100.076.621655
GTAGCTG80400.073.752766
TTGGGAG125050.068.603726
CTACTCG21850.067.355232
GCTGGGA170500.066.922719
TACTCGG22200.066.586663
GCTACTC50600.065.289841
CCCGAGT25200.065.161071
GCACTTT99100.063.319991
AAGTGCT90450.063.0641485
GGAGGCT137700.062.4904259
TAGCTGG98000.062.300677
TTTGGGA113950.061.7158055
ACTTTGG103250.061.178113
TCTACGA5100.00437476159.002216230-234
CACTTTG107100.058.2492562
Produced by FastQC (version 0.11.5)