FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3143588
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG9400.052.82332
AGTAGCT52150.047.9697535
TCGCGTT505.244504E-447.7636835
TACTCGG11200.046.9130063
CGAGTAG13000.046.8473933
GCTACTC25250.045.781771
GAGTAGC31150.045.235114
GTAGCTG53850.045.071766
CCGAGTA14050.044.7083132
TAGCTGG59150.043.1452037
TTGGGAG74400.042.6171346
GCTGGGA105000.042.264879
GGAGGCT71850.041.314629
CCCGAGT16900.040.4031031
GGGAGGC89450.039.304038
CGGAGGT13750.037.6397367
TCGGGAG15200.036.0059476
AAGTGCT62050.035.9863365
GGAGGTT39000.034.320058
CGTCGAG1051.4186147E-534.226191
Produced by FastQC (version 0.11.5)