FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3143588
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG12050.061.7442173
CCGAGTA13700.057.34822
CTACTCG13050.056.099762
GAGTAGC31850.055.3166164
TCGCGTT551.3090577E-554.1060835
TACTCGG13500.052.9079363
AGTAGCT53300.051.923575
TTGGGAG81250.051.1090856
GCTGGGA110450.050.5784959
GGAGGCT85900.049.658329
GTAGCTG56750.048.4330866
CCCGAGT16650.048.2936631
CTCGGGA15450.046.611785
GGGAGGC109100.045.096918
TAGCTGG64900.044.459357
AAGTGCT68350.043.6252565
TCGGGAG17700.043.3593256
CCCAAAG84350.042.367881
AAAGTGC70300.042.0798724
GTGCTGG72800.041.4326447
Produced by FastQC (version 0.11.5)