FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763295

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763295
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3843151
Sequences flagged as poor quality0
Sequence length35-351
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTCGA5203.0196225E-6135.11072320-329
CTCGATT6406.9769536E-493.69904340-345
GGCGCTT8300.002508122267.88509330-339
AGTAGCT91650.065.9857255
CGAGTAG23650.065.611443
TGTCGAT5501.21803554E-464.16908320-329
GAGTAGC61050.062.2402654
GTAGCTG96100.062.1061176
CCGAGTA24700.061.7949522
GCTGGGA180350.059.896039
GTCGATC3100.004977236557.12126320-329
GATTCGC3550.001268904556.307407340-345
TAGCTGG111300.055.1688967
TTGGGAG122650.054.3243146
CATACGT5800.00566572252.626545300-309
CGCAATA3400.003690046351.65998320-329
CCCGAGT30900.050.784151
CTACTCG19850.049.7545972
TACTCGG20450.048.8993263
AAGTGCT99400.048.5617685
Produced by FastQC (version 0.11.5)