FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763296

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3843151
Sequences flagged as poor quality0
Sequence length35-251
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGAA1250.00983255104.12511245
CTAAGCG4106.5447175E-495.23638245
CGAGTAG22550.071.979783
CCGAGTA22850.070.45252
AGTAGCT85350.068.740925
GTAGCTG89000.065.678756
GAGTAGC56050.064.68274
AACGTAA8151.2444482E-463.880436245
GCTGGGA182400.063.365229
TTGGGAG141900.061.8142176
CCCGAGT26950.061.5101931
TACTCGG24100.061.27783
CTATTCG4258.772986E-861.250057245
CTACTCG22750.061.1127932
TAGCTGG105900.056.6417357
AAGTGCT101400.056.4827085
GGAGGCT148000.056.2110339
GCACTTT113550.054.994881
TTTGGGA132900.054.4570665
ACTTTGG117700.053.4665263
Produced by FastQC (version 0.11.5)