FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763297

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763297
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3575122
Sequences flagged as poor quality0
Sequence length35-351
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT128200.091.658695
GTAGCTG133000.088.077486
CGAGTAG38100.087.4015963
CCGAGTA39550.085.244522
GAGTAGC90450.083.4040764
GCTGGGA267800.077.712569
TAGCTGG161500.074.692077
CCCGAGT45200.073.994741
AAGTGCT123950.072.375335
TTGGGAG169150.071.708526
TTTGGGA153400.067.29875
CTACTCG29350.066.943832
CCAAGTA56650.066.213322
TACTCGG29750.065.710553
CTGAGTA63650.065.245492
CAAGTAG55700.064.989043
AGTGCTG136900.064.663676
GCACTTT145400.064.396731
TGAGTAG63200.064.333413
GGAGGCT173900.063.0562329
Produced by FastQC (version 0.11.5)