FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763298

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763298
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3575122
Sequences flagged as poor quality0
Sequence length35-251
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT120800.092.071135
CGAGTAG35000.090.084493
CCGAGTA35700.088.318122
GTAGCTG125500.087.9836
GAGTAGC81700.085.778854
TTGGGAG187200.077.095856
GCTGGGA267250.076.670829
CCCGAGT42850.075.7508851
CTACTCG35500.073.386682
AAGTGCT125400.072.959555
GCACTTT153200.072.3218461
TAGCTGG159350.072.244627
GCTACTC79350.071.896161
TACTCGG36500.071.376093
TTTGGGA164100.071.242785
ACTTTGG156050.070.2442553
GGAGGCT196900.069.661469
CAAGTAG50150.068.611493
CACTTTG162000.067.924382
CTCGGGA40300.067.079675
Produced by FastQC (version 0.11.5)