FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2847976
Sequences flagged as poor quality0
Sequence length35-351
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAACG1553.5695673E-4104.61848300-309
AGTAGCT83300.078.935625
CGTAACG1302.6402404E-577.292885330-339
CGAGTAG22300.076.223793
GTAGCTG86700.074.384836
CCGAGTA22800.073.174052
GAGTAGC55050.071.962294
CGAGTAA2850.005263352870.25586330-339
CTATCGG1800.006337456468.46814260-269
GCTGGGA175250.066.43239
TAGCTGG100850.066.291737
TAAGTCG2652.662673E-765.976036310-319
TTGGGAG110200.064.956756
AAGTGCT83900.063.7622955
CTACTCG17300.062.7235642
TAGGGCG3402.9122511E-662.571632340-345
CCCGAGT27350.061.808881
GCTACTC42450.060.773571
CAAGTAG37800.059.47963
TACTCGG18550.059.1415673
Produced by FastQC (version 0.11.5)