FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3378840
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAACG600.001266795139.931171
CGCGACT757.877405E-539.9311681
CGAGTAG9150.037.1946833
CCGAGTA9750.034.973312
CGTCGAG1554.803951E-934.778761
CCCGAAT5900.033.5015751
CCCGAGT12250.032.7598571
GAGTAGC22050.031.4101564
GCTGGGA68500.030.1348699
ACGCGTA400.005369264629.74406884-85
AGTAGCT35950.029.2290785
TAGCTGG40750.029.1966347
CGGAGGT9000.029.0830927
TTGGGAG54950.029.0133256
GCTACTC19850.028.0624581
TACTCGG7450.028.0503943
GGGAGGC59250.028.0121368
GGAGGCT54400.026.9008879
ACCGCGC3400.026.4250371
CTACTCG7250.026.4045332
Produced by FastQC (version 0.11.5)