FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3378840
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA9150.041.6325072
CGAGTAG8800.040.5828253
TACTCGG8900.038.7892763
CTACTCG8950.037.907532
CCCGAGT12600.036.3968661
GCTGGGA70100.034.2871489
GGCGGAG16100.034.0087435
CGGAGGT10950.033.68577
GCTACTC20550.033.6194041
TTGGGAG60050.033.486656
GGGAGGC69750.033.2650648
GGAGGCT59250.033.035099
AGGCGGA16500.032.8269964
TGGGAGG74350.030.4868097
GAGTAGC23750.030.3246254
AGTAGCT40150.030.0911755
GGAGGTT33500.030.0130338
GCGGAGG14300.029.9546646
TCGGGAG12800.029.281856
TAGCTGG45150.029.2525147
Produced by FastQC (version 0.11.5)