FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3106631
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG13700.070.816582
CGAGTAG13850.069.190113
TACTCGG14250.067.665623
CCGAGTA14650.065.411812
GCTACTC34400.061.2983171
AGTAGCT58650.057.538035
GAGTAGC37000.057.1076854
CCCGAGT18150.055.7925221
GCTGGGA120400.055.29469
GTAGCTG62650.054.984046
TTGGGAG91250.053.072646
GGAGGCT99850.050.5273979
CTCGGGA19200.049.907365
TAGCTGG73450.049.4912647
TCGGGAG20600.049.0976646
ACTCGGG18800.048.75634
AAGTGCT71850.047.795725
AAAGTGC72950.047.7307744
CCCAAAG89400.046.3740921
GCACTTT74300.046.2584081
Produced by FastQC (version 0.11.5)