FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3808130
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG2200.038.143431
CGAGTAG10350.034.04423
CTACTCG8950.033.437192
TACTCGG9050.032.3354533
CGGAGGT10300.031.764987
GGGAGGC70750.031.0258058
TTGGGAG66350.030.7522136
CCGAGTA11700.030.6936242
GCTGGGA79750.029.01639
GGAGGCT64200.029.0022989
GCTACTC25200.028.3048441
AGTAGCT43250.028.1666685
CTCGCGT1704.2894862E-728.10284
GAGTAGC26250.027.2998664
TGGGAGG79650.027.0362177
CCCGAGT16350.026.3954521
GGAGGTT34550.026.17098
TAGCTGG49700.025.7338777
CTCGGGA11000.025.5150595
GTAGCTG45950.025.3742776
Produced by FastQC (version 0.11.5)