FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763309

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763309
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3655027
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG13600.052.6750833
CCGAGTA14300.048.517952
GAGTAGC35350.043.3993034
AGTAGCT57150.042.6140865
CCCGAGT17450.042.209491
TTGGGAG86350.040.7153746
GTAGCTG59100.040.2629746
GCTGGGA114650.039.122869
CTACTCG11300.038.6389122
TACTCGG11750.037.5973053
TAGCTGG66550.036.9177557
GGGAGGC104650.036.6651768
GCTACTC29300.035.9704171
GGAGGCT83600.035.8639079
AAGTGCT69650.033.8520665
GCACTTT71050.033.4600751
TGGGAGG113200.032.4244087
AGTGCTG74800.032.2892346
CGGAGGT17000.032.1937987
CCCAAAG94650.031.9500261
Produced by FastQC (version 0.11.5)