FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4195225
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG13350.051.2712552
TACTCGG14150.049.635013
CGGAGGT20450.047.71197
GCTACTC32200.043.84791
CGAGTAG14800.041.422673
GCTGGGA112200.040.9875959
GGAGGCT91600.040.0733349
CCGAGTA15750.040.0573922
TTGGGAG89350.039.818316
AGTAGCT58250.039.2353135
GAGTAGC35800.038.9041024
CTCGGGA18150.038.36645
GGGAGGC113950.037.6958778
GGCGGAG27100.037.3354575
TCGGGAG19700.037.1462556
AGGCGGA26800.037.088924
ACTCGGG19550.036.2295344
CCCGAGT18900.035.9334831
TAGCTGG65550.035.6694157
GGAGGTT54650.035.2716568
Produced by FastQC (version 0.11.5)