FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763314

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763314
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3137810
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG12600.062.3544123
CTACTCG13250.061.0923462
CCGAGTA13550.058.421962
GCTACTC30800.058.4081231
GAGTAGC30400.056.974854
TACTCGG14100.054.4545753
AGTAGCT50350.053.068225
TTGGGAG86550.051.20976
CCCGAGT17300.050.960381
GTAGCTG52250.050.6683466
GCTGGGA112050.049.5373579
GGAGGCT87450.048.2335789
GCACTTT72200.044.8826751
GGGAGGC113850.044.7827578
CTCGGGA18150.044.2634055
TAGCTGG62300.043.5451557
TCGGGAG19150.043.4932946
ACGCGAT558.5164927E-443.287363
ACTTTGG74450.042.53153
AAGTGCT69350.041.9614075
Produced by FastQC (version 0.11.5)