FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4178152
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG11500.045.547452
TACTCGG11250.044.4432683
CGGAGGT18300.043.559557
GCTACTC29700.040.721581
CCGAGTA12900.040.6043172
GCTGGGA100600.037.1356779
CGAGTAG12700.036.556963
TTGGGAG83200.035.6074526
GGAGGCT87200.035.2698639
AGGCGGA25550.034.944564
GGCGGAG25550.034.9403765
GAGTAGC33250.034.5497474
GCGGAGG23350.034.393936
TCGCGTT700.002786353534.0086335
GGGAGGC104600.033.781938
AGTAGCT53850.033.7086525
GGAGGTT50800.033.6084028
CCCGAGT18650.033.5425071
GTAGCTG55950.033.1733556
TAGCTGG62050.032.596197
Produced by FastQC (version 0.11.5)