FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3404504
Sequences flagged as poor quality0
Sequence length35-351
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG22200.073.783143
AGTAGCT84500.069.034185
TAGGGCG3403.1666168E-668.98407340-345
GAGTAGC55050.067.9202044
GTAGCTG87500.066.317516
CCGAGTA25050.064.957942
CGTGTCG3507.933785E-563.391647330-339
GCTGGGA173450.060.311749
TTGGGAG115650.057.3515556
TAGCTGG102100.057.1620677
CCCGAGT30500.054.9603921
AAGTGCT87750.054.3140685
CTACTCG17400.052.5550232
CACCGGC13650.007452938751.548534340-345
TTTGGGA111400.050.616865
GCACTTT99900.050.406491
TACTCGG18050.049.836543
GCTACTC42450.049.638311
AGTGCTG95500.049.548816
GGAGGCT118400.049.292639
Produced by FastQC (version 0.11.5)