FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3897899
Sequences flagged as poor quality0
Sequence length35-251
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGAA700.0017093953253.69864245
TACGCGT1650.0076513947141.33096240-244
ACATACG6551.0004442E-1083.13782230-234
CATACGT5353.1432137E-971.473915230-234
CTCGCAA4600.002386736268.75463225-229
CTACTCG23500.068.224122
CGAGTAG19950.068.050383
GCTACTC55350.064.974871
AGTAGCT81600.064.3268365
TACTCGG24850.063.9974753
GAGTAGC52350.062.6102454
CCGAGTA22000.062.0031742
CGCAATA2755.8625246E-661.952972220-224
TTGGGAG135250.061.7855956
GTAGCTG84700.060.4321146
GCTGGGA181550.059.3060729
TCGGGAG30900.058.56336
GCACTTT107800.056.9423941
GGAGGCT148400.056.9196329
AAGTGCT97600.056.232085
Produced by FastQC (version 0.11.5)