FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3281958
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG17700.066.8060763
GAGTAGC44800.061.1831974
CCGAGTA19500.060.4357072
AGTAGCT70250.059.4166835
GTAGCTG73050.056.108156
CTACTCG13650.056.0969622
CCCGAGT22500.054.57821
TACTCGG13500.054.4120863
GCTGGGA144750.052.4806569
TAGCTGG82400.051.0409937
GCTACTC32200.048.1827741
TTGGGAG95200.047.6151286
AAGTGCT73550.046.8458025
GGAGGCT98000.044.3729449
TCGGGAG20950.043.1605036
GGGAGGC127050.042.8423588
GCACTTT76700.042.3303451
CCCAAAG95900.042.2880741
CCAAAGT86950.042.2435842
AGTGCTG82700.042.2240146
Produced by FastQC (version 0.11.5)