FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3281958
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG15650.072.647772
TACTCGG15750.069.918883
CGAGTAG17350.068.960413
GCTACTC38650.066.2715151
CCGAGTA18050.064.966932
AGTAGCT65750.061.7351265
GAGTAGC42450.060.7174454
TTGGGAG104400.057.4431536
CCCGAGT21400.056.2270771
GTAGCTG71950.055.2362176
GCTGGGA144750.054.7885559
GGAGGCT117950.054.3749739
GCACTTT82550.051.8823621
ACTCGGG21300.051.4211164
AAGTGCT80450.049.345045
CTCGGGA22750.048.9219675
TAGCTGG83550.048.706617
ACTTTGG86750.048.512453
CCAAAGT90750.048.4731332
CCCAAAG101650.048.2867241
Produced by FastQC (version 0.11.5)