FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4330237
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG14700.056.2721522
CGAGTAG15300.054.8433153
TACTCGG15700.053.825093
CCGAGTA16600.053.057852
GCTACTC37400.046.6668621
CCCGAGT21100.046.016941
GAGTAGC37150.045.1737674
CGGAGGT25750.044.5908437
GCTGGGA129450.044.579519
TTGGGAG104000.044.3909076
GGAGGCT107700.043.6944669
AGTAGCT63100.042.247365
GGGAGGC140100.041.0208478
CTCGGGA21700.040.8580325
TCGGGAG23050.040.5223246
GGAGGTT64550.040.2764178
GTAGCTG66050.040.2624666
TAGCTGG74050.037.5195357
ACTCGGG22700.037.227044
GGCGGAG34000.036.9278835
Produced by FastQC (version 0.11.5)