FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3847901
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG2750.043.5439831
CGAGTAG9750.034.2926063
CCGAGTA10400.032.2018172
GCTGGGA88450.031.1399049
CGGAGGT12250.031.0797827
GAGTAGC28950.030.934484
AGTAGCT49600.030.0921215
TACTCGG10650.029.7127843
GTAGCTG50950.029.4232376
CCCGAGT14850.028.626141
CTACTCG10450.028.0417732
GGAGGCT68950.027.7815029
TAGCTGG56000.027.7259627
CTCGGGA12450.026.8542565
TTGGGAG68750.026.131746
GCTACTC27650.025.9846551
GGCGGAG17750.025.8992255
TCGGGAG13500.025.5580866
GGGAGGC79600.025.4097258
GCGGAGG17150.023.934216
Produced by FastQC (version 0.11.5)