FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3847901
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG11650.043.430243
CTACTCG12000.042.1635252
CCGAGTA12350.041.932572
CGAGTAG11800.041.8692633
CGGAGGT15050.041.506397
GCTACTC28350.039.5082781
CTCGGGA14500.036.9431765
TCGGGAG15550.036.728966
GGAGGCT75300.035.7903029
TTGGGAG68900.035.661426
CCCGAGT16450.035.1308941
GGCGGAG21350.035.1262975
GAGTAGC30650.034.1807374
GCTGGGA93250.033.7496269
GGGAGGC90650.033.208168
AGGCGGA21550.033.1461564
AGTAGCT49200.031.9373235
GCGGAGG19400.031.2799646
GGAGGTT44750.030.8430358
ACTCGGG16800.030.8254344
Produced by FastQC (version 0.11.5)